Degradation of phospholipid and release of diglyceride-rich membrane vesicles during protoplast formation in certain gram-positive bacteria.
نویسنده
چکیده
Membrane phospholipid was found to be hydrolyzed presumably by an intracellular phospholipase C, and diglyceride-rich membrane vesicles were released from the cells during protoplast formation in Bacillus cereus Bacillus subtilis, Micrococcus lysodeikticus, and Staphylococcus aureus. The released membranes consisted mainly of small vesicles of 50 to 100 nm in diameter. They have a lower density than that of protoplast membranes in all the bacteria tested in the present study.
منابع مشابه
A novel mechanism for the biogenesis of outer membrane vesicles in Gram-negative bacteria
Bacterial outer membrane vesicles (OMVs) have important biological roles in pathogenesis and intercellular interactions, but a general mechanism of OMV formation is lacking. Here we show that the VacJ/Yrb ABC (ATP-binding cassette) transport system, a proposed phospholipid transporter, is involved in OMV formation. Deletion or repression of VacJ/Yrb increases OMV production in two distantly rel...
متن کاملNew insight into the application of outer membrane vesicles of Gram-negative bacteria
This review presents a brief outline of our current knowledge of the structure and chemical composition of the outer membrane vesicles (OMVs), originating from the surface of Gram negative bacteria including their outer membrane proteins and lipopolysaccharides. Moreover, the functional roles and applications of OMVs in medical research such as OMV-based vaccines, OMV adjuvants properties, OMV ...
متن کاملجداسازی وزیکولهای ترشحی غشاء خارجی باکتری اشرشیاکلی با استفاده از فیلتراسیون با جریان مماسی
Background: Among pathogenic bacteria, Gram-negative bacteria are more important. The secreting outer membrane vesicles of the bacteria are important role in physiology, virulence, and the interaction between host and pathogen. These vesicles allow bacteria to transmit enzyme and its contents as conserved form and also these involved in viability. Since they are represented as bacteria candidat...
متن کاملInvestigating the Antimicrobial Efficacy of Liposomal Vancomycin in Gram-positive and Gram-negative bacteria- A Preliminary Mechanistic Study
Outer membrane of Gram-negative bacteria is a permeability barrier to many antibacterial agents, including the glycopeptide antibiotics such as vancomycin hydrochloride and as a result these antibiotics are ineffective against Gram negative bacteria. Different strategies have been described to overcome such limitation, including application of nanoparticles, as was shown in our previous studies...
متن کاملImmunogenicity of enterotoxigenic Escherichia coli outer membrane vesicles encapsulated in chitosan nanoparticles
Objective(s): Enterotoxigenic Escherichia coli (ETEC) is an important cause of diarrheal disease in humans, particularly in children under 5 years and travelers in developing countries. To our knowledge, no vaccine is licensed yet to protect against ETEC infection. Like many Gram-negative pathogens, ETEC can secrete outer membrane vesicles (OMVs). These structures contain various immunogenic vi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of bacteriology
دوره 121 3 شماره
صفحات -
تاریخ انتشار 1975